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. 2019 Dec 27;23(1):100807. doi: 10.1016/j.isci.2019.100807

Figure 2.

Figure 2

Spermidine Inhibits IFN-DC Activation

BM monocytes were cultured with GM-CSF and IL-4 for 7 days. MoDCs were sorted and treated with 2,000 U/mL IFN-α for 24 h, and then the IFN-DCs were stimulated with 10 μg/mL R837 in the presence or absence of spermidine (SPD) for 24 h.

(A) The protein levels of TNF-α, IL-6, and IL-12/23 p40 (n = 3).

(B) Flow cytometry analysis of MHCII, CD80, and CD40 (n = 3). Data are representative of three independent experiments. Error bars represent SEM. P values were determined by two-tailed unpaired t test *p < 0.05, **p < 0.01, ***p < 0.001.