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. 2019 Dec 27;23(1):100807. doi: 10.1016/j.isci.2019.100807

Figure 3.

Figure 3

Spermidine Enforces Metabolic Quiescence in IFN-DCs

BM monocytes were cultured with GM-CSF and IL-4 for 7 days. MoDCs were sorted and treated with 2,000 U/mL IFN-α for 24 h, and then the IFN-DCs were stimulated with 10 μg/mL R837 in the presence or absence of spermidine (SPD) for 24 h.

(A) The extracellular acidification rate (ECAR) (left) and oxygen consumption rate (OCR) (right) were detected (n = 4).

(B) Statistics of glycolysis and maximum OCR are shown (n = 4). Data are shown as mean ± SEM. P values were determined by two-tailed unpaired t test *p < 0.05, **p < 0.01, ***p < 0.001. n.s., not significant.

(C) MoDCs or IFN-DCs were treated with IFN-α, R837, or R837 and spermidine for the indicated times, respectively. The levels of protein were analyzed by immunoblot. β-ACTIN was used as the internal loading control. Data are representative of three independent experiments.