Figure 4.

Motility metrics compared in a 3D environment in vitro to in vivo. (a) From left to right the images represent the in vitro invasion assay, live imaging micrograph from cells in a 3D hyaluronan matrix in vitro and glioma cells implanted in mouse brain at the tumor border with invasive cells beyond the border (arrowheads). (b) in vitro percent invasion, (c) percent cells migrating, (d) speed, (e) chemotactic index, (f) total displacement, and g) net displacement graphed by glioma cell line versus the number of invaded cells beyond the tumor border in vivo per mm² of tissue. Pearson r correlation with p values listed on each graph