Figure 3.

Adoptive transfer of T_regs leads to engraftment after immunomodulation in young, prediabetic NOD mice. A: Experimental scheme. B: Percentage of Thy1.1⁺ donor T_reg engraftment among the total gated CD4⁺Foxp3⁺ T cells in peripheral blood. C: Number of donor T_regs/1 × 10⁶ CD45⁺ lymphocytes in peripheral blood. D: Percentage of Thy1.1⁺ donor T_reg engraftment in the total gated CD4⁺Foxp3⁺ T cells 4 weeks post–T_reg infusion in SP, LN, pLN, and pancreas (PAN) of young, female prediabetic NOD mice receiving αCD3+T_reg, αCD3+T_reg+IAC, αCD3+CyP+T_reg, or αCD3+CyP+T_reg+IAC. IAC = anti–IL-2 (JES6)+rmIAC. E: Percentage of Thy1.1⁺ donor T_regs in the gated CD4⁺Foxp3⁺ T cells in mice receiving the αCD3+CyP+T_reg+IAC regimen and CD4⁺Foxp3⁺ in the gated total CD4 T cells in mice receiving the αCD3 regimen in the peripheral blood. F: Percentage of CD4⁺Foxp3⁺ in the gated CD45⁺ population. G: CD4⁺Foxp3⁺ in the gated CD4 T cells in the peripheral blood of young, prediabetic female NOD mice (aged 5–6 weeks) in NOD mice receiving αCD3, αCD3+IAC, αCD3+CyP, αCD3+T_reg, αCD3+T_reg+IAC, αCD3+CyP+T_reg, or αCD3+CyP+T_reg+IAC. n = 5–6 mice per group. *P < 0.0001; +P < 0.001; @P < 0.01; ^P < 0.05. B and C: Multiple Student t test compared with αCD3+T_reg. F and G: Two-way ANOVA followed by Dunnett multiple comparison test compared with αCD3. d, day; rmIAC, recombinant murine IAC.