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A) G-/F-actin spin-down assay of peripheral blood mononuclear cell (PBMC) from MCI donors. Spin-down assay was performed on PBMC obtained from donors listed in Table 3 as described in the Materials and Methods. B) Analysis of white blood cells (WBC) from postmortem blood sample of a 65-year-old DS donor. WBCs were isolated and processed for G-/F-actin spin-down assay similarly as described above. Samples of S fraction from Fragile X (FX) and control (CTR) are included for comparison.
