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. 2020 Jan 21;20:1. doi: 10.1186/s12896-019-0592-9

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — The PH_(1–110) GFP particles are purified and separated by size in a single centrifugation step. a Sucrose gradients (right) in which the separation of the particles is observed after centrifugation. With the SDS-PAGE (left) it can be seen that PH_(1–110) GFP particles highly pure particles are recovered in each gradient. b Confocal microscopy shows the presence of PH_(1–110) GFP particles of different sizes obtained in each sucrose gradient. c With the NTA equipment the sizes of the particles and the concentration of each particle were measured by 1 mL of solution. The peaks of the curves show the populations of particles. Dotted lines indicate the SD