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. 2020 Jan 21;20:1. doi: 10.1186/s12896-019-0592-9

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — The PH_(1–110) GFP particles are stable after 1 year at room temperature. PH_(1–110) GFP particles that were maintained under different temperature conditions during: a 1 month, b 3 months, c 6 months and d 1 year were injected into mice and IgG antibodies specific to GFP were measured. All groups were compared against the group of mice immunized with particles maintained at − 70 °C. RTD = Room Temperature Dehydrated, RT = Room Temperature. Error bars indicate the means ± SD (n = 5). * p < 0.05; ** p < 0.01; *** p < 0.001; ns = not significant. (Two-way ANOVA with Dunnett post-tests)