Fig. 6.

A rescue assay was further performed to confirm that FOXO3 was the functional target of miR-10b-3p. a-b The mRNA and protein levels of FOXO3 in KYSE150 and KYSE450 cell lines cotransfected with miR-10b-3p mimic and pEGFP-C1 plasmid containing FOXO3 CDS sequence. c The cell growth curve was measured by MTS cotransfected with miR-10b-3p mimic and FOXO3 plasmids in KYSE 150 cell lines, and the OD 570 was normalized to the star point (0 h). d Transwell assay of cells cotransfected with miR-10b-3p mimic and FOXO3 plasmids. e The expression of FOXO3 at the mRNA and protein level post siRNA silencing in KYSE150 cells. f Representative images and quantification of transwell assay after the transfection of FOXO3 siRNA into the KYSE150 cell lines. Each experiment was performed in triplicate. Data are presented as the mean value ± SD. ^●, P < 0.05; ^●●, P < 0.01