Fig. 1.

Example high resolution respirometry trace showing the rate of oxygen flux (red line) over time after sequential titration of substrates and inhibitors, expressed as pmol s⁻¹ mg⁻¹. The blue line represents oxygen concentrations within the chamber (nmol ml⁻¹). Vertical dashed lines represent timepoints where substrates have been added. The horizontal black lines which overlay the red line plateaus indicate the position of datapoints. ADP, adenosine diphosphate; CI, complex I; CII, complex II; CIV, complex IV; ETS, electron transport system; FCCP, carbonyl cyanide 4-(trifluoromethoxy)-phenylhydrazone; OXPHOS, oxidative phosphorylation; ROX, residual oxygen flux; TMPD, N,N,N′,N'-tetramethyl-p-phenylenediamine.