Figure 3.

Forskolin promotes long‐term rhythms in PER2::LUC expression. Whole area bioluminescence rhythms prior to and following forskolin treatment for the (Aa) SFO and (Ab) OVLT, demonstrating long‐lasting synchrony and clear oscillations up to 14 days post‐forskolin treatment. Individual cellular rhythms revived and synchronized post‐forskolin treatment in the (Ba) SFO and (Bb) OVLT. C, Phase clustering of individual cells post‐forskolin treatment; synchrony did not vary between the structures. D, Damping rate relative to the first peak after forskolin treatment (day 0) showed no change between the structures. E, The mean period of single‐cell oscillations did not change pre‐ and post‐forskolin treatment, nor did change the F, variability in these individual oscillator periods. Duration of forskolin treatment is denoted by the gray‐filled bar. Data in C and D plotted as mean ± SEM