Figure 5.

PKK depletion results in enhanced expression of multiple innate immune pathways in lungs of uninfected mice. Mice were treated subcutaneously with PKK ASO or control ASO twice weekly for 3 weeks and euthanized 4 days after final dosing. RNA was purified from whole lungs and genome‐wide mRNA expression was analyzed. (A) Volcano plots [integrating adjusted P values and fold‐expression (log₂ fold‐change)] depicting the global alteration in gene expression after treatment with PKK ASO relative to control ASO administration. Horizontal line indicates Benjamini–Hochberg (BH) adjusted p < 0.05. Red dots denote overexpressed genes; turquoise dots indicate underexpressed genes. (B) Ingenuity pathway analysis of elevated transcripts (red bars) with Benjamini–Hochberg (BH) adjusted Fisher's p < 0.01 demarcating significance; pathway analysis did not reveal significantly altered pathways for underexpressed genes. (C) Heatmap representation of transcript expression (rows) grouped as Ingenuity canonical signaling pathways in PKK ASO‐ and control ASO‐treated animals. Red, high expression; turquoise, low expression.