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f ‐HM‐SiR, a fluorogenic and spontaneously blinking fluorophore for bioorthogonal DAinv. a) Spiro‐cyclization equilibrium of spontaneously blinking and fluorogenic f ‐HM‐SiR and its Diels–Alder cycloadduct (DAinv‐Product). b) Fluorescence enhancement of f ‐HM‐SiR upon conversion in DAinv at pH 4 in aqueous buffer. c) pH‐dependent fluorescence of f ‐HM‐SiR (blue) and DAinv‐Product (red).
