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. 2020 Jan 16;12:363–373. doi: 10.2147/CMAR.S230171

Figure 5.

Figure 5

GBP1 is a direct target of miR-199b-5p. (A) Supposed miRNA binding sites in GBP1 sequences. (B) Direct target sites as verified by dual-luciferase reporter gene assay. (C) GBP1 expression in cervical cancer cell lines (SW756, C-33A, SiHa, HeLa and CaSki) and normal human cervical epithelial cell line HcerEpic based on qRT-PCR. (D) Western Blot for protein level of GBP1 in HcerEpic, HeLa and C-33A cell lines. (E) GBP1 expression in cervical cancer tissues and normal tissues on TCGA dataset. (F) The correlation between miR-199b-5p and GBP1 on TCGA dataset. *P<0.05, **P<0.01, ***P<0.001.