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. 2020 Jan 16;12:363–373. doi: 10.2147/CMAR.S230171

Figure 6.

Figure 6

LINC01783/miR-199b-5p axis is critical for GBP1 expression. (A) Transfection of MiR-199b-5p inhibitor with or without LINC01783 siRNA into HeLa cells and qRT-PCR evaluation for RNA level of GBP1. (B) Western blot of GBP1 protein level after treatment of HeLa cells, GAPDH as the control. (C) Transfection of C-33A cells with miR-199b-5p mimics with or without LINC01783 overexpression plasmid and relative RNA levels of GBP1 as detected by qRT-PCR. (D) Relative protein level of GBP1 for transfection with miR-199b-5p mimics and reversion by LINC01783 expression plasmid. (E) Relative RNA level of GBP1 for transfection with LINC01783 MUT overexpression plasmid or LINC01783 WT overexpression plasmid. (F) Relative protein level of GBP1 for transfection with LINC01783 WT overexpression plasmid or LINC01783 MUT overexpression plasmid. **P<0.01, ***P<0.001.

Abbreviation: ns, no significant difference.