Figure 1.

Fucose distribution over time following intraperitoneal (IP) injection. A, Confirmation of K‐fucose kit (megazyme) specificity, showing detection of increasing concentrations of l‐fucose, d‐mannose and d‐galactose compared with manufacturer's l‐fucose standard. B, l‐fucose measurements in serum and kidney following a single IP injection of 100 mg of l‐fucose, at 5, 10, 20, 25 and 30 min and then 15‐min intervals to 60 minutes and 120 and 240 min. C, l‐fucose measurements in serum and kidney samples following a second IP injection of 100 mg l‐fucose (given 60 min after the first dose) at the listed time points following the second dose, up to 60 min. Kidneys were homogenized and l‐fucose concentration was determined in homogenized lysate and serum. Kidney values are adjusted to weight of kidney. Each data point is representative of at least two biological repeats and three technical repeats. Error bars on all graphs are standard error of the mean