Figure 7.

RACE pretest and intersection miRNAs targeting Smad2, PKCα, and TCONS_00020456 lncRNA. (a) Top. Transcript ratio assessment of TCONS_00020456. ACTB and MALAT1 were used as references. The length of the ACTB amplification products was 110 base pair (bp), MALAT1 was 150 bp, and TCONS_00020456 was 521 bp. Down, Specificity evaluation of TCONS_00020456 transcription products. The figure on the right shows the position of the detected primers. Above is the electrophoresis pattern of PCR products using a temperature gradient. The four temperature gradients used are 58, 60, 62, and 65°C. The marker diagram is shown on the right. (b) A three‐set Venn diagrams presenting the intersection miRNAs that might target Smad2, PRKCα, and lncRNA. List1 is the possible miRNAs targeting Smad2, List2 is the predicted miRNAs that might target lncRNA, and List 3 is the potential miRNAs targeting PKCα. The intersections are as follows: hsa‐miR‐502‐3p, hsa‐miR‐501‐3p, hsa‐miR‐1321, hsa‐miR‐302e, hsa‐miR‐1279, hsa‐miR‐1272, hsa‐miR‐1248, hsa‐miR‐320d, hsa‐miR‐320c, hsa‐miR‐320b, hsa‐miR‐320a, hsa‐miR‐636, and hsa‐miR‐513a‐5p. (c) NCBI BLAST results for lncRNA TCONS_00020456 and has‐miR‐1279. lncRNA, long noncoding RNA; miRNA, microRNA; PCR, polymerase chain reaction; PKCα, protein kinase C α