Figure 2.

Closed culture vessel and circuit modules. (a‐1) Closed culture vessels in assembled and disassembled states. These are quoted from Hitachi Review (Shu et al., 2016). (a‐2) Cross‐section through a closed culture vessel. The culture dish and temperature‐responsive cell culture insert are set in the vessel, and the culture medium and gas are exchanged via the port. (b) Circuit diagram of the closed system circuit. By switching valves, it is possible to pump the culture medium and gas to any location