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. 2020 Jan 21;10:889. doi: 10.1038/s41598-020-57803-3

Figure 7.

Figure 7

Genic and protein expression in thoracic aortas from wild-type and CD mice. (A) qPCR mRNA expression of Limk1, a gene contained in the Williams-Beuren syndrome critical region, and Adra1a (α1A-adrenoceptors). Results are the mean ± SEM from wild-type (n = 4) and CD (n = 5) mice. *P < 0.05; ***P < 0.01 by Multiple t-test with Sidak-Bonferroni. (B) Western blot analysis for nNOS protein expression. Bar graphs show the results of densitometric analyses from pooled data for nNOS, iNOS, and eNOS protein expression. The molecular weight (kDa) of the protein is shown on the right side of the blot. Total protein was used as a loading control. Results are the mean ± SEM from wild-type (n = 5) and CD (n = 5) mice. *P < 0.05 by Mann-Whitney U test. (C) Representative photomicrographs and quantification of immunofluorescence (red) for nNOS, iNOS, and eNOS in confocal microscopic aortic sections. Scale bar, 20 μm. Results are the mean ± SEM from wild-type (n = 8) and CD (n = 8) mice. *P < 0.05 by Student’s t-test.