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. 2020 Jan 21;10:882. doi: 10.1038/s41598-020-57677-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of cycling hypoxia on the abundance of the phosphorylated form of STAT1, p65 and c-jun as well as on the abundance of IRF5 in human M0, M1 and M2 macrophages. THP-1 M0, M1 and M2 macrophages were exposed to normoxia (N), chronic hypoxia (chH) or cycling hypoxia (cyH) for 1h30 (A), for 3 h (B) or for 6 h (C), and then total protein extraction was performed. Abundance of the phosphorylated form of STAT1 (Tyr701), p65 (Ser536) and c-jun (Ser63) as well as the total abundance of IRF5 was detected by western blotting (n = 3). α-tubulin was used as loading control. Fluorescence intensity of each immunoblotted protein was quantified and normalized for α-tubulin (D–G). Statistical analysis was performed by two-way ANOVA (or one-way ANOVA for P-STAT1 in M1 macrophages) and Holm-Sidak test as post hoc test. *P < 0.05; **P < 0.01; ***P < 0.001.