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. 2020 Jan 21;10:842. doi: 10.1038/s41598-020-57673-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mitochondrial ROS mediated CRIF1 deficiency-induced eNOS uncoupling in HUVECs. (A) Comparison of total biopterin, BH₂ + B and BH4 content in control and cells with or without various amount of MitoTEMPO and CRIF1 siRNA. (B) BH4 synthesis enzymes (GCH-1, PTS, SPR, and DHFR) expression in control and CRIF1-deleted HUVECs pretreated with MitoTEMPO (1 mM or 10 mM). (C) Measurement of band density was used to analysis quantification of protein expression (n = 3 per group; *P < 0.05, vs. control; ^#P < 0.05, vs CRIF1 siRNA 100 pmol).