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. 2020 Jan 21;10:828. doi: 10.1038/s41598-020-57663-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Proteomic analysis of young and aged astrocyte EV proteome. (A) Top 25 differentially expressed proteins (as listed by gene name) following quantitative analysis of protein abundance between young and aged astrocyte-derived EVs. (B) Differential expression and hierarchical cluster analysis between replicates using protein-wise exact tests. An FDR cutoff of 0.01 was used to identify significantly differentially expressed proteins.