Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jan 21;10:854. doi: 10.1038/s41598-020-57817-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Schematic diagrams of live-cell imaging and subsequent embryo transfer. We performed IVF to obtain pronuclei (PN) stage embryos. After injecting mRNA encoding histone H2B-mCherry into the cytosol of 2PN, time-lapse imaging was performed 3-dimensionally. We observed chromosome segregation during the 1^st, 2^nd, and 3^rd mitosis. Single embryo transfer of observed blastocysts was performed on day 4. Six blastocysts with different coat colors were implanted as controls to determine whether the case of no pups was due to embryo quality or pseudopregnant mouse implantation ability/technique of embryo transfer. Sixteen days after embryonic transfer, pups were obtained by cesarean section.