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. 2020 Jan 21;10:854. doi: 10.1038/s41598-020-57817-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Categorization of severity and type of chromosome segregation error. The categorization of chromosome segregation error based on imaging. There are two types of chromosome segregation errors: misalignment at metaphase (a) and lagging chromosome at anaphase (b). Severity of chromosome segregation errors were categorized as follows, 1: normal chromosome segregation (NCS), 2: one or more chromosomes deviate a few micrometers from others and return, 3: one or more chromosomes deviate completely from others and return, 4: one or more chromosomes deviate completely from others and form the micronucleus. The typical snapshots of each severity of misaligned and lagging chromosomes are shown in (c) and (d). Bar = 50 μm.