Fig. 3. Coordinated expression of decidual immune surveillance genes.

a Decidual gene network B2 annotated to highlight genes implicated in uNK-cell activation and immune surveillance of senescence cells. b Primary EnSC cultures were decidualized with 8-bromo-cAMP and MPA (C+M) for the indicated days. ELISAs were performed on spent medium collected at 48 h intervals to examine secreted levels of CXCL14, IL-15 and TIMP-3. Grey dotted lines indicate secreted levels in individual cultures (n = 4). Black solid lines indicate the median level of secretion. c Four independent primary EnSC cultures were decidualized with 8-bromo-cAMP and MPA (C+M) for the indicated days. ELISAs were performed on spent medium collected at 48 h intervals to examine secreted levels of clusterin (CLU) and sST2. Cultures established from the same biopsy are colour matched between plots (dotted lines). Black solid lines indicate the median level of secretion. d Schematic representation of uNK-cell co-culture experiments. A total of 5000 primary uNK cells were co-cultured for 48 h from the indicated timepoint with 50,000 decidualized cells seeded in 96-well plates. Four independent EnSC cultures were used. e SAβG activity in undifferentiated and decidualized (C+M) cells co-cultured with or without uNK cells. Four independent EnSC cultures were used. f Secretion of CLU in decidualized (C+M) cells co-cultured with uNK cells relative to decidualized cells cultured without uNK cells. g Secreted sST2 levels in the same culture. Four independent EnSC cultures were used; lines connect paired samples.