Table 3.

Sequences of gene-specific primers and probes.

Gene	Nucleotide sequences
SNCA NM_000345.3	Probe: 5′-VIC-TGTTCTCTATGTAGGCTCCAA-BHQ2-3′ Forward Primer: 5′-AGCAGGAAAGACAAAAGAGG-3′ Reverse Primer: 5′-TTGCTCTTTGGTCTTCTCAG-3′
VCP NM_007126.3	Probe: 5′- VIC-CAACAGTGTGGTGTCCTTGTCC-BHQ2-3′ Forward Primer: 5′-ATCGGTTAATTGTTGATGAA-3′ Reverse Primer: 5′- GTCTCTTCTTTCCTTTCAG-3′′
AARS NM_001605.2	Probe: 5′-FAM-TATGTTCACTCGTCTGCCACCATCCCAT-BHQ1-3′ Forward Primer: 5′- CGGCAGCGATTTATAGATTTCTTCAAG-3′ Reverse Primer: 5′- GGTGAGATGGGTCAATTGTGTTCA-3′
PSMD7 NM_002811.4	Probe: 5′-FAM-GCTTCTGTAGGCAGCCCTAGGTCCTTCGG-BHQ1-3′ Forward Primer: 5′-TGTCCTAATTCCGTATTGGTCATCATTG-3′ Reverse Primer: 5′-CGTGTTCAAATGTTTTCGAGGTTGG-3′
Vcp NM_009503.4	Probe: 5′-VIC-CTTTGAACTCCACAGCACGCAT-BHQ2-3′ Forward Primer: 5′-CCATCCGTAAAGGAGATA-3′ Reverse Primer: 5′-GAGCAACAATACAGTAAGG-3′
Bcat2 NM_001243053.1	Probe: 5′-FAM-CGGATACACTCCAACAGCTCCTGCTTGT-BHQ1-3′ Forward Primer: 5′-TCAACATGGACAGGATGCTACG-3′ Reverse Primer: 5′-CCAGTCTTTGTCTACTTCAATGAGC-3′
Psmd7 NM_010817.2	Probe: 5′-FAM-AGTCCTAGGTCCTTTGGCTTCACGTCGA-BHQ1-3′ Forward Primer: 5′-CTGCACAAGAATGATATCGCCATC-3′ Reverse Primer: 5′-CTCCACTGAGATGTAGGCTTCG-3′

*Accession numbers in GenBank database. FAM and VIC, fluorescent dyes; BHQ1 and BHQ2, quenchers of fluorescence.