Table 2.
Primers and related information of the candidate reference genes and the target gene.
| Gene | Primer pairs (5′-3′) | Efficiency (%) | Coefficient of determination (r2) | product size (bp) |
|---|---|---|---|---|
| GAPDH |
F: GTATTGGCCGTCTGGTCCT R: GGGAGACCTCACCGTTGTAA |
98.0 | 0.997 | 144 |
| 18S |
F: GTGGAGCGATTTGTCTGGTT R: CTCAATCTCGTGTGGCTGAA |
96.5 | 1.000 | 130 |
| ACTB |
F: CATGTACGTTGCCATCCAAG R: ACCAGAGGCATACAGGGACA |
104.9 | 0.996 | 138 |
| EF1A |
F: CGGCCACCTGATCTACAAGT R: GCCTTCAGTTTGTCCAGCA |
90.9 | 0.997 | 123 |
| CTSD |
F: GAAGAAGGTGGAGCAGAACG R: TGCGGGTGATGTTGATGTAG |
96.8 | 0.999 | 137 |
| B2M |
F: GGCAGTTCCATCTGACCAAG R: ATGTTTGACTCCCAGGCGTA |
92.8 | 0.998 | 112 |
| ERα |
F: GCCACCACTATCTGGAAACC R: CCTGACTCCCCCAAACTGTA |
90.9 | 0.996 | 115 |