Table 3.
Ct values of candidate reference genes expressed in hypothalamus, pituitary, ovary and liver during ovarian development of turbot.
| Tissues | Stages | GAPDH | 18S | ACTB | EF1A | CTSD | B2M |
|---|---|---|---|---|---|---|---|
| Hypothalamus | Prevtg | 23.30 | 9.72 | 21.30 | 19.03 | 17.45 | 22.37 |
| Evtg | 20.76 | 10.89 | 22.86 | 21.05 | 20.47 | 19.98 | |
| Latvtg | 27.14 | 10.48 | 23.84 | 19.97 | 19.98 | 19.65 | |
| Mig-nucle | 23.68 | 11.95 | 24.45 | 21.08 | 20.10 | 19.15 | |
| Atre | 25.44 | 11.5 | 23.69 | 21.05 | 20.65 | 18.65 | |
| Pituitary | Prevtg | 24.46 | 10.12 | 23.01 | 19.60 | 19.71 | 18.00 |
| Evtg | 22.68 | 12.02 | 23.71 | 21.55 | 20.09 | 17.46 | |
| Latvtg | 20.10 | 10.85 | 23.26 | 20.03 | 20.74 | 19.91 | |
| Mig-nucle | 23.57 | 11.91 | 24.99 | 19.88 | 21.67 | 19.75 | |
| Atre | 19.80 | 9.73 | 23.00 | 18.70 | 19.27 | 15.77 | |
| Ovary | Prevtg | 18.98 | 8.85 | 18.04 | 11.57 | 16.95 | 16.24 |
| Evtg | 15.03 | 8.09 | 16.87 | 11.71 | 16.50 | 17.98 | |
| Latvtg | 15.66 | 8.70 | 17.06 | 10.56 | 17.07 | 17.89 | |
| Mig-nucle | 17.00 | 8.31 | 18.44 | 13.49 | 18.56 | 17.81 | |
| Atre | 17.18 | 9.57 | 19.62 | 14.22 | 18.35 | 18.70 | |
| Liver | Prevtg | 26.91 | 7.86 | 21.74 | 18.18 | 22.55 | 17.57 |
| Evtg | 26.18 | 7.00 | 20.15 | 19.27 | 23.45 | 18.37 | |
| Latvtg | 24.17 | 7.75 | 22.53 | 20.97 | 20.70 | 16.96 | |
| Mig-nucle | 26.31 | 8.26 | 23.44 | 18.92 | 23.76 | 18.92 | |
| Atre | 24.82 | 7.57 | 23.50 | 16.26 | 22.41 | 16.26 |
Prevtg: previtellogenesis; Evtg: early vitellogenesis; Latvtg: late vitellogenesis; Mig-nucle: migratory nucleus; Atre: atresia.