Table 4.

Standard deviations (SD) and correlation coefficient (r) of the reference genes based on their quantification cycle values analyzed by Bestkeeper.

Tissue	Factor	18S	GAPDH	B2M	EF1A	ACTB	CTSD
Hypothalamus	SD	0.69	1.78	0.97	0.75	0.92	0.91
	r	0.96	—	—	0.95	0.91	0.93
Pituitary	SD	0.83	1.74	0.77	0.67	0.61	0.73
	r	0.95	—	0.82	0.71	0.83	0.86
Ovary	SD	0.38	1.14	0.59	1.23	0.84	0.78
	r	0.79	—	0.58	—	0.93	0.83
Liver	SD	0.28	0.94	0.82	0.78	1.06	0.82
	r	0.48	0.71	0.94	0.60	—	0.71

Note: Standard deviations above 0.95 are grayed and discarded from the calculation of correlation coefficient. Lower values of correlation coefficient indicate least stably expressed genes, and higher values of correlation coefficient indicate more stably expressed genes.