Fig. 2. Transient PU.1 expression induces a myeloid expression program.

a Schematic of the experimental setup. b Immunoblotting confirming the translation and nuclear location of full-length PU.1 in transfected cells using increasing amounts of IVT mRNA. c Relative mRNA expression levels of ETS family members in PU.1-expressing or control cells measured by RNA-seq (n = 2 each). d Example tracks for a PU.1-activated gene. Coverage represents the mean of n = 3 or n = 2 experiments for ATAC/ChIP-seq or RNA-seq data, respectively. e Volcano plot of significantly PU.1 up- or downregulated genes (red or blue, respectively). Genes with known function in myeloid or B-cell biology are labeled. f Metascape-derived network of Gene Ontology (GO) terms associated with PU.1-induced genes in CTV-1 cells. The significance of enrichment of a particular term is depicted with the log10 of the p-value and indicated by node coloring. g Comparison of median expression levels of PU.1-induced genes (threefold) with PU.1 expression levels across various human blood cell types data based on CAGE (Cap Analysis of Gene Expression) expression data from the FANTOM Consortium³⁵. Related cell types in the hematopoietic tree share similar colors. The Pearson’s correlation as well as the corresponding p-value are indicated. b, c, e–g Source data are provided as a Source Data file.