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. 2020 Jan 21;11:402. doi: 10.1038/s41467-019-13960-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Design of PU.1-deletion mutants. The DNA-binding domain (ETS domain) was kept in all constructs and a 3 × -FLAG-tag was added N-terminal for detection. b Immunoblot confirming the nuclear expression of PU.1-deletion mutants compared with full-length PU.1 in transfected cells using IVT mRNA. c IGV genome browser tracks of the CD84 locus showing PU.1 ChIP-seq coverage of CTV-1 cells transfected with varying amounts of PU.1 IVT mRNA as well as PU.1 mutant mRNA. ATAC-seq coverage of CTV-1 cells transfected with PU.1 (blue) and mutPU.1 (turquoise) IVT mRNA is depicted in the two bottom rows. d Two-dimensional visualization of the distribution of the indicated samples across annotated PU.1 peaks using tSNE embedding. Replicates of the same mRNA transfections are indicated by coloring. e Differential ChIP-seq peaks of WT vs. less PU.1 (15%, light blue), WT vs. ΔAQP (red), WT vs. ΔQ (green), and WT vs. ΔA (brown), as well as differential peaks between ΔQ vs. ΔA (brown) are shown across ATAC-seq-derived PU.1 peak clusters (introduced in Fig. 3). b–e Source data are provided as a Source Data file.