Table 1.

Optimized MRM transitions of AQC-derivatised chiral amino acids.

Molecular Species	Q1	Q3	DP	EP	CE	CXP	Time
Lys	487	171	71	10	41	10	100
Orn	473	171	81	10	41	10	100
Trp	375	171	61	10	33	20	100
Tyr	352	171	116	10	31	10	100
Cit	346	171	11	10	37	18	100
Arg	345	171	46	10	41	26	100
Phe	336	171	11	10	29	14	100
His	326	171	36	10	25	22	100
Met	320	171	96	10	29	18	100
Glu	318	171	81	10	29	10	100
Gln	317	171	56	10	35	10	100
Asp	304	171	76	10	29	22	100
Asn	303	171	81	10	25	14	100
Ile	302	171	86	10	27	16	100
allo-Ile	302	171	86	10	27	16	100
Leu	302	171	86	10	27	16	100
Cys	292	171	71	10	27	10	100
Thr	290	171	61	10	27	20	100
allo-Thr	290	171	61	10	27	20	100
Val	288	171	91	10	27	18	100
Pro	286	171	61	10	25	16	100
Ser	276	171	61	10	27	18	100
d4-Ala	264	171	1	10	27	18	100
Ala	260	171	1	10	27	18	100
Gly	246	171	51	10	27	18	100

Multiple Reaction Monitoring: MRM; Q1 Mass: Precursor ion (m/z); Q3 Mass: Product ion (m/z); De-clustering Potential: DP (V); Entrance Potential: EP (V); Collision Energy: CE (V); Collision Cell Exit Potential: CXP (V); Time (msec).