Figure 3.

The incapability of hexamminecobalt(III) chloride (CoHex) to inhibit the transport of Zn²⁺ by proteoliposomes with (a) TmCorA, (b) MjCorA and (c) ZntB. Black—25 μM Zn²⁺ with no added CoHex, red— 25 μM Zn²⁺  + 1 μM CoHex, blue—25 μM Zn²⁺  + 10 μM CoHex, green—25 μM Zn²⁺  + 50 μM CoHex, magenta—empty liposomes with 25 μM Zn²⁺  + 50 μM CoHex. All substrates were simultaneously added at 1 min. Higher concentrations of CoHex led to the collapse of proteoliposomes (see Supplementary Fig. 1). Error bars represent s.e.m. from three or more technical replicates of independent batches of proteoliposomes.