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. 2020 Jan 21;10:840. doi: 10.1038/s41598-020-57869-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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transport competition assays with Co²⁺ and Mg²⁺ in the proteoliposomes, loaded with FluoZin-1, with (a) MjCorA and (b) TmCorA, (black—100 μM CoSO₄ with no added MgSO₄, red—100 μM CoSO₄ + 100 μM MgSO₄, blue —100 μM CoSO₄ + 200 μM MgSO₄, green—100 μM CoSO_4 +500 μM MgSO₄, light blue—100 μM CoSO₄ + 1000 μM MgSO₄, magenta—empty liposomes with 100 μM CoSO₄ + 1000 μM MgSO₄). All substrates were simultaneously added at 1 min. Error bars represent s.e.m. from three or more technical replicates of independent batches of proteoliposomes.