Table 1.
Strains and plasmids used in this study.
| Strains and Plasmids | Relevant characteristics | Source or reference |
|---|---|---|
| E. coli | ||
| BL21 Star (DE3) | Overexpression of proteins | Novagen |
| Salmonella | ||
| SJW1103 | Wild type for motility and chemotaxis | 61 |
| TH8426 | ∆fliK | 47 |
| MMK1012 | fliK(∆206–235) | This study |
| MMK1013 | fliK(∆206–245) | This study |
| MMK1014 | fliK(∆206–255) | This study |
| MMK1015 | fliK(∆206–265) | This study |
| Plasmids | ||
| pTrc99AFF4 | Modified pTrc expression vector | 62 |
| pKM002 | pTrc99AFF4/FliK | 48 |
| pNM201 | pTrc99AFF4/FliK(∆2–99) | 44 |
| pMMK521 | pETDuet-1/FliK(I304amber) + FlhBC | 45 |
| pMMK1001 | pTrc99AFF4/FliK(∆206–210) | This study |
| pMMK1002 | pTrc99AFF4/FliK(∆211–215) | This study |
| pMMK1003 | pTrc99AFF4/FliK(∆216–220) | This study |
| pMMK1004 | pTrc99AFF4/FliK(∆221–225) | This study |
| pMMK1005 | pTrc99AFF4/FliK(∆226–230) | This study |
| pMMK1006 | pTrc99AFF4/FliK(∆231–235) | This study |
| pMMK1007 | pTrc99AFF4/FliK(∆206–215) | This study |
| pMMK1008 | pTrc99AFF4/FliK(∆216–225) | This study |
| pMMK1009 | pTrc99AFF4/FliK(∆226–235) | This study |
| pMMK1010 | pTrc99AFF4/FliK(∆206–220) | This study |
| pMMK1011 | pTrc99AFF4/FliK(∆221–235) | This study |
| pMMK1012 | pTrc99AFF4/FliK(∆206–235) | This study |
| pMMK1013 | pTrc99AFF4/FliK(∆206–245) | This study |
| pMMK1014 | pTrc99AFF4/FliK(∆206–255) | This study |
| pMMK1015 | pTrc99AFF4/FliK(∆206–265) | This study |
| pMMK1015SP | pTrc99AFF4/FliK(∆206–265SP) | This study |
| pMMK1016 | pTrc99AFF4/FliK(∆2–99 + ∆206–210) | This study |
| pMMK1017 | pTrc99AFF4/FliK(∆2–99 + ∆211–215) | This study |
| pMMK1018 | pTrc99AFF4/FliK(∆2–99 + ∆216–220) | This study |
| pMMK1019 | pTrc99AFF4/FliK(∆2–99 + ∆221–225) | This study |
| pMMK1020 | pTrc99AFF4/FliK(∆2–99 + ∆226–230) | This study |
| pMMK1021 | pTrc99AFF4/FliK(∆2–99 + ∆231–235) | This study |
| pMMK1022 | pTrc99AFF4/FliK(∆2–99 + ∆206–215) | This study |
| pMMK1023 | pTrc99AFF4/FliK(∆2–99 + ∆216–225) | This study |
| pMMK1024 | pTrc99AFF4/FliK(∆2–99 + ∆226–235) | This study |
| pMMK1025 | pTrc99AFF4/FliK(∆2–99 + ∆206–220) | This study |
| pMMK1026 | pTrc99AFF4/FliK(∆2–99 + ∆221–235) | This study |
| pMMK1027 | pTrc99AFF4/FliK(∆2–99 + ∆206–235) | This study |
| pMMK1028 | pTrc99AFF4/FliK(∆2–99 + ∆206–245) | This study |
| pMMK1029 | pTrc99AFF4/FliK(∆2–99 + ∆206–255) | This study |
| pMMK1030 | pTrc99AFF4/FliK(∆2–99 + ∆206–265) | This study |
| pMMK1030SP | pTrc99AFF4/FliK(∆2–99 + ∆206–265SP) | This study |
| pMMK1031 | pETDuet-1/FliK(∆2–99 + I304amber) + FlhBC | This study |
| pMMK1032 | pETDuet-1/FliK(∆2–99 + ∆206–265 + I304amber) + FlhBC | This study |
| pMMK1032SP | pETDuet-1//FliK(∆2–99 + ∆206–265SP + I304amber) + FlhBC | This study |