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. 2020 Jan 21;10:787. doi: 10.1038/s41598-019-57409-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Sequence analysis and functional annotation of assembled unigenes identified from L. vannamei hemocytes under NLHS and NH conditions with and without VP_AHNPD challenge. (A) Number of BLAST hits (E-value < 10) in the reference transcriptome and sequence distribution in different organisms. (B) Number of sequences with hits according to level 2 gene ontologies (GO). (C) Number of sequences in the transcriptome assembly that are similar to the Cluster of orthologous groups (COGs). Letter codes with detailed descriptions are listed beneath the graph. (D) Top 20 KEGG pathways mapped in the assembly.