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. 2020 Jan 21;10:787. doi: 10.1038/s41598-019-57409-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Relative expression analysis of miRNAs in response to VP_AHPND infection following the NLHS and NH treatments in L. vannamei hemocyte. Total small RNAs from hemocyte of VP_AHPND-infected L. vannamei under NH- and NLHS-treated conditions which are NH-VP and NLHS-VP, respectively, were used as templates for specific stem-loop first strand cDNA synthesis. Relative expression levels of 10 miRNAs (lva-miR-7170-5p, lva-miR-2169-3p, lva-miR-184, lva-miR-92b-5p, lva-miR-317, lva-miR-4901, lva-miR-92a-3p, lva-miR-61, lva-miR-2898, and lva-miR-6090) were determined by RT-qPCR and normalized against U6, the internal reference, at 0, 6, and 24 hpi. The bar graphs are data from RT-qPCR presented as means ± standard deviations and triangles (▲) are data from the small RNA-Seq. The results were derived from triplicate experiments. Asterisks indicate significant differences (P < 0.05) from the respective VP_AHPND-infected shrimp at 0 hpi.