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. 2020 Jan 21;10:777. doi: 10.1038/s41598-019-57275-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Semi-quantitative analysis score of active caspase-3 in spleen of wild-type (WT), FcGRIIb−/− and pristane mice with or without 60 days of dextran sulfate solution (DSS) in drinking water (A) were demonstrated. In addition, the quantitative flow-cytometric analysis of splenocyte from these mice in term of necrotic cells (propidium iodide, PI +ve) (B), early apoptosis cells (Annexin V +ve, PI −ve) (C), late apoptosis cells (Annexin V +ve, PI +ve) (D), apoptotic macrophage in spleen (Annexin V +ve, F4/80 +ve) (E) and apoptotic B cell in spleen (Annexin V +ve, B220 +ve) were indicated (n = 4–6 per group).