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. 2020 Jan 21;10:766. doi: 10.1038/s41598-020-57698-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of SENP2 is downregulated in bortezomib resistant MM patients: (A) Differential gene expression analysis using GSE5900 dataset identified 1214 down-regulated genes in MM patient samples (n = 12) compared to healthy controls (n = 22). SENP2 is indicated as a highly downregulated gene with in MM patients compared to healthy donors. (B) Schematic representation of sgRNA based bortezomib resistance screening assay. (C) The Heat maps representing next generation sequencing readouts of 126 bortezomib resistant sgRNA library clones before and after bortezomib treatment. SENP2 enrichment is indicated, which had the highest enrichment ratio among the 126 bortezomib resistant sgRNA library clones. (D) The next generation sequencing enrichment scores for the selected top seven sgRNA clones identified in bortezomib resistance screening assay. Among them, SENP2-sgRNA clones were found to have highest enrichment score. (E) RNA expression analysis of SENP2 from MM patient’s CD138 + bone marrow cells, bortezomib resistance (n = 4) and bortezomib sensitive (n = 8). (F) Western blot analysis to detect SENP2 protein expression levels in bortezomib resistance (n = 4) and bortezomib sensitive (n = 4) MM patient’s CD138 + bone marrow cells. Here, GAPDH serves as a loading control.