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. 2020 Jan 21;10:812. doi: 10.1038/s41598-020-57733-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of neurovascular coupling by LSI/EEG. (A) RMS power/flux overlay of representative data from a littermate control mouse demonstrating IHR after SE onset. Periods of IHR are denoted between vertical blue lines. (B) RMS/flux overlay of representative data from a TRPC3smcKO mouse showing more sustained blood flow with less variability during late SE. The three sharp dips in the flux data are artifacts corresponding to the sequential injection of methylscopolamine, pilocarpine and pentylenetetrazol (as described in Fig. 3 legend), when the camera view was briefly occluded by the experimenter’s hand administering the injection. The camera was intentionally blocked to provide a clear marker on the flux trace of injection times.