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. 2020 Jan 21;10:887. doi: 10.1038/s41598-020-57820-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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A Characterisation of HLSC. (A) Undifferentiated HLSC grown in chamber slides were stained for immunofluorescence analysis of Albumin, Alpha-fetoprotein, Vimentin, Nestin, Oct4, SSEA-4, CK18, CK19. Nuclei were stained with DAPI. Secondary antibody controls are shown. (B) Immunofluorescence analysis of HLSC differentiated in bioscaffold. HLSC differentiated for 15 days in bioscaffold were stained for the presence of the hepatocyte-specific markers, Albumin and Cytochrome 1a1. Nuclei were stained with Hoechst. Arrowheads show double-positive cells. Secondary antibody controls are shown.