Figure 8.

Role of Ca²⁺ in GSK‐induced ATP release from GP bladder tissue. A, Removal of Ca²⁺ from Tyrode's superfusate significantly reduces GSK‐stimulated (1 μM) ATP release (Median, [25%, 75%], n = 8, Mann‐Whitney)); control: 166 ± 55 pmoles/g tissue/min. B(i), GSK (1 μM) generates a Ca²⁺ transient (left) in a freshly isolated single urothelial cell from GP bladder; ATP‐induced Ca²⁺ transient (right). B(ii), Averaged data; GSK (1 μM) evokes a significant rise in [Ca²⁺]_i, (mean ± SEM, n = 7, P < .05). B(iii), Averaged data; GSK (1 µM) induced [Ca²⁺]_i rise (n = 9) vs ATP (100 µM) induced[Ca²⁺]_I, (n = 14); P < .05, Mean ± SEM, unpaired t test