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. 2019 Sep 23;116(12):3228–3241. doi: 10.1002/bit.27159

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© 2019 The Authors. Biotechnology and Bioengineering Published by Wiley Periodicals, Inc.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Expansion of human iPSCs in the BioBLU 3c and spinner flask. (a) Representative phase‐contrast aggregate images were taken during iPSC expansion. iPSCs formed aggregates which grew from Day −3 to 0 in both the spinner flask and bioreactor. (b) The average aggregate size was shown to be similar between the BioBLU 3c and spinner flask. (c) Viable cell density and percent viability during expansion showed similar growth kinetics. (d) Flow cytometry analysis of undifferentiated iPSCs on Day 0 of the cardiomyocyte induction process showed high expression of key pluripotency markers SSEA‐4, Tra‐1–81, Tra‐1–60, and Oct4 in both spinner flask and bioreactor (pink: target, blue: isotype). Scale bars represent 200 μm. iPSC, induced pluripotent stem cells [Color figure can be viewed at wileyonlinelibrary.com]