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. 2019 Sep 24;235(4):3320–3328. doi: 10.1002/jcp.29220

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© 2019 The Authors. Journal of Cellular Physiology Published by Wiley Periodicals, Inc.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Tumor cells isolated from ascitic fluid and solid tumor. (a) Isolated cells from ascitic fluid with spindle‐shaped growth. (b) Isolated cells from ascites fluid, where cellular variability is observed. (c) Isolated cells from solid tumor in DMEM/F12 medium supplemented with SFB 10%. (d) Isolated cells from ascites liquid in DMEM/F12 medium supplemented with SFB 10%. All photos were taken with inverted microscope objective ×20. DMEM, Dulbecco's modified Eagle medium [Color figure can be viewed at wileyonlinelibrary.com]