Table 4.

Functional profiling of Bi-valent VHHs.

	hFractalkine Competition		cyFractalkine Competition		Chemotaxis		Inhibition of Fractalkine Internalization
Bi-valent VHH	Percent Block	IC50 (nM)	Percent Block	IC50 (nM)	Percent Block	IC50 (nM)	Percent Block	IC50 (nM)
54A12-35GS-54A12 (BI 16)	102	0.19	98	0.69	88	2.0	90	0.8
54D05-35GS-54D05 (BI 17)	99	0.31	95	1.6	89	3.0	91	0.6
66B02-35GS-66B02 (BI 18)	102	0.03	97	0.10	98	0.6	92	0.33
66G01-35GS-66G01 (BI 19)	100	0.29	96	0.82	85	2.0	89	0.90