Figure 3.

Effect of BST2 silencing on the invasion of HTR-8/SVneo cells treated with IFN-γ. HTR-8/SVneo cells (0.1 × 10⁶/well) were seeded in 6-well culture plate and cultured overnight at 37°C in 5% CO₂ and 70% relative humidity. The next day, cells were transfected with scrambled siRNA or BST2 siRNA and subsequently used to study their invasion by matrigel matrix invasion assay as described in Materials and Methods. Silencing of BST2 was confirmed by qRT-PCR and Western blotting. Panels a and b show transcript and protein levels of BST2 in scrambled siRNA and BST2-silenced cells, respectively, on treatment with and without IFN-γ (10 ng/mL). The representative blots of BST2 and GAPDH from one of the three experiments are appended with Panel b. Each bar represents relative expression after normalization with 18S rRNA or GAPDH and expressed as mean ± SEM of three independent experiments. Panel c shows the fold change in the invasion of cells transfected with scrambled or BST2 siRNA, respectively, followed by treatment with and without IFN-γ for 24 h. The representative images of the cells in the invasion assay are also appended with Panel c. The results are expressed as mean ± SEM of three independent experiments. Fold change in invasion is expressed with respect to scrambled siRNA-transfected cells without treatment with IFN-γ.