Figure 4.

Spreading of BST2-silenced HTR-8/SVneo spheroids on the monolayer of Ishikawa cells. HTR-8/SVneo cells (0.1 × 10⁶/well) were seeded in 6-well cell culture plate overnight at 37°C in 5% CO₂ and 70% relative humidity. The next day, cells were transfected with BST2 siRNA and scrambled siRNA and kept for 48 h followed by the formation of spheroids using 2500 cells/spheroid as described in Materials and Methods. The histogram shows the fold change in the area covered by individual scrambled siRNA-transfected HTR-8/SVneo spheroids and BST2-silenced HTR-8/SVneo spheroids, respectively, in the presence/absence of IFN-γ after 24 h. Each bar represents a relative area of spreading after normalization with untreated scrambled siRNA-transfected HTR-8/SVneo spheroids. The representative images of the spheroids are appended. Scale bar represents 20 μm. Values are expressed as mean ± SEM of three independent experiments.