Figure 5.

Expression of E-cadherin after BST2 silencing in HTR-8/SVneo cells treated with IFN-γ. HTR-8/SVneo cells (0.1 × 10⁶/well) were cultured overnight in 6-well culture plate at 37°C in 5% CO₂ and 70% relative humidity and subsequently transfected with BST2 and scrambled siRNA. The scrambled siRNA- and BST2 siRNA-transfected cells were used to study the expression of E-cadherin by Western blotting using a mouse monoclonal antibody against E-cadherin as described in Materials and Methods. The bar graph shows the expression of E-cadherin at protein level in scrambled siRNA-transfected and BST2-silenced cells, respectively, on treatment with and without IFN-γ (10 ng/mL) and the representative blots of E-cadherin and GAPDH from one of the three experiments are appended. Each bar represents relative expression after normalization with GAPDH with respect to untreated scrambled siRNA-transfected cells. Values are expressed as mean ± SEM of three independent experiments.