Figure 6.
Degradation of CyaR by ArcZ. MG1655 cells were co-transformed with pACyaR or pACyaRmA and pArcZ or pArcZmC. IPTG was added into the culture 2 h after a 1/100 dilution of the culture (grown at 37°C) and the culture was further incubated for 2 h. Cellular levels of CyaR and ArcZ were measured by Northern blot analysis; 5S rRNA was detected as a loading control. V, vector control; CyaR, pACyaR (a derivative of pAKA); CyaRmA, pACyaRmA (a derivative of pAKA); ArcZ, pArcZ (a derivative of pHMB1); ArcZmC, pArcZmC (a derivative of pHMB1).