Figure 2.

Evaluation of drug-induced readthrough over GLA nonsense mutations.

(A) Representative Western blotting analysis on full-length rGal variants resulting from treatment of transiently transfected HEK293 cells with G418. For rGal detection, wild-type lysate was further diluted (1:20) and used as reference. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as control. Mock, cells transfected with the gutted vector; wt, wild-type rGal. (B) ELISA-based analysis of full-length rGal variants transiently expressed in HEK293 cells treated with G418. Results, indicated as % of wild-type rGal, are reported as mean ± standard deviation from three independent experiments. In both analyses, the full-length rGal arising from nonsense variants was not observed in untreated conditions.