Figure 2.

Peak shapes for different mAbs (B) eluted from a ¹D ProA column using different buffers (A). (a) NISTmAb peak shapes obtained using three different ProA elution buffers. Buffer A was 12 mM hydrochloric acid and 150 mM NaCl at pH 1.9. Buffer B was 25 mM potassium phosphate and 150 mM potassium chloride at pH 2.5. Buffer C was 100 mM potassium phosphate and 100 mM potassium chloride at pH 3.0. (b) ProA elution profile of three mAbs using elution buffer C. Dashed lines indicate the portion on of the effluent that would be captured using a 120 µL sampling loop.